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1) After a PCR run using a new set of primers your gel

/in /by

1) After a PCR run using a new set of primers your gel shows no
amplification bands at all. What are some possible parameters with your reaction which might contribute to a non-successful amplification?
a. Low annealing temperature
b. High annealing temperature
c. Long annealing step
d. Short annealing step
e. Long extension step
f. Short extension step

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